“Breath analysis in cancer diagnosis: state of art and future perspectives”
The odor of body excretions, such as breath, sweat or urine, has been for centuries used by physicians for disease diagnosis and specific odors have been associated with different pathological disorders. Nowadays it’s realized that pathological disorders are usually accompanied by alterations in human metabolism and production of specific volatile metabolic odorants, not characteristic for healthy person. However, disordered metabolism can also produce odorless metabolites that can only be detected by instrumental analysis and different instrumental approaches have been developed for the analysis of body excretions and in particular of human breath. The breath analysis is non-invasive and thus very attractive for future clinical applications and preventive screening of population for early disease detection that substantially increases the chances of successful treatment, e.g. in case of cancer. In the present webinar the current activities and achievements in breath analysis for clinical applications will be reviewed, different instrumental techniques and analytical approaches used will be discussed as well as still existing limitations impeding wide application of breath analysis in clinical practice.
Nowadays different, competing techniques and methods have been developed by different vendors and research groups, comprising mass spectrometry based methods with indirect (different GC-MS methods) or direct breath analysis (Proton Transfer Reaction MS, Selected Ion Flow Tube Mass Spectrometry, Ion Mobility Spectrometry) and clinical differentiation based on quantitative or qualitative analytical approaches. The indirect methods are coupled with different methods of breath sampling and metabolites pre-concentration methods.
The special attention is nowadays also given to modern statistical methods of data treatment to produce valid and robust conclusions from acquired experimental data. Thus, seminar attendees will get a general overview of the breath analysis research.
Assoc. Prof. Andreas K. Tsakalof
Laboratory of Biochemistry, Faculty of Medicine, University of Thessaly, Larisa, Greece.
Dr. Andreas Tsakalof is nowadays Associate Professor of Medicinal Chemistry at the Faculty of Medicine, University of Thessaly. He graduated from Moscow State University of Fine Chemical Technologies named after M.V.Lomonosov in 1977 and earned his PhD in chemistry from Russian Academy of Science, Nesmeyanov Institute of Organoelement Compounds in 1986. In 1998 he was awarded Mary Curie individual fellowship as experienced researcher and received funded position at the University of Surrey, UK for the period 1998-1999. During this period he was trained in molecular toxicology and simultaneously attended lectures in MSc Program in Toxicology of the Surrey University. Andreas Tsakalof is European Registered Toxicologist and Vise-President of the Hellenic Society of Toxicology.
Professor A.Tsakalof is the author of 57 research publications in peer reviewed journals and his publications received more than 1427 citation and 22 h-factor (Scopus data on 02-2019, Author ID: 6602277700). He is also the author of three patents and two book chapters.
Nowadays, Professor A.Tsakalof’ research activities and interests are focused on:
1. GC-MS analysis of VOC in exhaled air for disease diagnosis
2. Aapplication of chromatography and chromatography-mass spectrometry in clinical chemistry and toxicology, human biomonitoring,
3. Vitamin D in human health and disease,
4. Fabrication of molecularly imprinted polymers and their application for the identification and isolation of bioactive molecules.
He is the leader and scientific supervisor of the research groups involved in these research activities.